8GXM
HUMAN SUGP1 433-586
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-11-02 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 61 |
| Unit cell lengths | 60.160, 60.160, 177.768 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.950 - 2.810 |
| R-factor | 0.2252 |
| Rwork | 0.217 |
| R-free | 0.29710 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 8gxl |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.129 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX (1.17.1_3660) |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.910 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Number of reflections | 8896 | 887 |
| <I/σ(I)> | 27.5 | |
| Completeness [%] | 99.8 | |
| Redundancy | 7.8 | |
| CC(1/2) | 0.999 | 0.870 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 0.1 M Bis-Tris (pH 7.5), 25% (w/v) polyethylene glycol 3350. |
