8GXL

HUMAN SUGP1 433-577

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-29
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9792
Spacegroup name	P 61
Unit cell lengths	59.657, 59.657, 178.404
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	51.660 - 2.400
R-factor	0.2044
Rwork	0.198
R-free	0.26100
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	0.935
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX (1.17.1_3660)
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	51.660	2.490
High resolution limit [Å]	2.400	2.400
Number of reflections	14002	1405
<I/σ(I)>	16.9
Completeness [%]	99.9
Redundancy	5.2
CC(1/2)	0.997	0.835

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	0.2 M ammonium tartrate dibasic, pH 7.0, and 20% (w/v) polyethylene glycol 3350.