8GHJ
Crystal structure of human AQP2 T125M mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MAX IV BEAMLINE BioMAX |
Synchrotron site | MAX IV |
Beamline | BioMAX |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-01-24 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.977 |
Spacegroup name | P 42 |
Unit cell lengths | 118.310, 118.310, 90.400 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.500 - 3.900 |
R-factor | 0.2903 |
Rwork | 0.288 |
R-free | 0.31310 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4nef |
RMSD bond length | 0.003 |
RMSD bond angle | 0.870 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.17_3644) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.990 |
High resolution limit [Å] | 3.900 | 3.900 |
Number of reflections | 22380 | 1641 |
<I/σ(I)> | 0.43 | |
Completeness [%] | 99.9 | |
Redundancy | 21.3 | |
CC(1/2) | 0.990 | 0.287 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 20- 30% PEG400, Tris-HCl pH 8.5, 0.1M MgCl2, 0.1M NaCl, 0.025 M CdCl2 added to the drop only |