8G4Y
Structure of ZNRF3 ECD bound to peptide MK1-3.6.10
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 2.0.1 |
Synchrotron site | ALS |
Beamline | 2.0.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-05-06 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.036 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 135.551, 35.226, 48.856 |
Unit cell angles | 90.00, 109.11, 90.00 |
Refinement procedure
Resolution | 32.020 - 1.410 |
R-factor | 0.2145 |
Rwork | 0.213 |
R-free | 0.23610 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 0.916 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.160 | 1.640 |
High resolution limit [Å] | 1.410 | 1.550 |
Rmerge | 0.056 | 0.748 |
Rmeas | 0.062 | 0.834 |
Rpim | 0.028 | 0.366 |
Total number of observations | 22697 | |
Number of reflections | 35592 | 4445 |
<I/σ(I)> | 14.4 | 2.5 |
Completeness [%] | 97.3 | |
Redundancy | 5 | 5.1 |
CC(1/2) | 0.998 | 0.782 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 277 | 0.1 M sodium citrate pH 4.5, 20% PEG 4000 plus silver bullet A5 (Hampton Research) |