8G0P
Crystal structure of the human Ndc80:Nuf2 loop region
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-06-23 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.97910 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 49.903, 72.259, 80.065 |
Unit cell angles | 90.00, 98.56, 90.00 |
Refinement procedure
Resolution | 40.750 - 2.000 |
R-factor | 0.262 |
Rwork | 0.260 |
R-free | 0.29680 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.003 |
RMSD bond angle | 0.456 |
Data reduction software | xia2 |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.750 | 1.870 |
High resolution limit [Å] | 1.840 | 1.840 |
Rmerge | 0.122 | 4.030 |
Rmeas | 0.132 | 4.440 |
Rpim | 0.050 | 1.820 |
Number of reflections | 36742 | 1005 |
<I/σ(I)> | 9.5 | 2.4 |
Completeness [%] | 98.7 | 84.5 |
Redundancy | 6.8 | 5.7 |
CC(1/2) | 1.000 | 0.330 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 20% (w/v) polyethylene glycol 4000, 0.1 M TRIS pH 8.6, and 0.2 M magnesium chloride |