8FSX

Crystal structure of hen egg white lysozyme

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SEALED TUBE
Source details	OXFORD DIFFRACTION ENHANCE ULTRA
Temperature [K]	125
Detector technology	CCD
Collection date	2021-01-15
Detector	OXFORD TITAN CCD
Wavelength(s)	1.54056
Spacegroup name	P 43 21 2
Unit cell lengths	77.416, 77.416, 37.219
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	13.410 - 1.600
R-factor	0.24168
Rwork	0.239
R-free	0.28684
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.545
Data reduction software	HKL-2000
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	13.410	1.630
High resolution limit [Å]	1.600	1.600
Rmerge	0.125	1.352
Rmeas	0.140	1.609
Rpim	0.060	0.859
Total number of observations	74950	2237
Number of reflections	15278	729
<I/σ(I)>	7	0.8
Completeness [%]	99.0
Redundancy	4.9	3.1
CC(1/2)	0.934	0.387

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	4.6	295	1.2 M NaCl, 100 mM Sodium Acetate, 50 mg/mL Lyzozyme, 30% Glycerol for cryoprotection