8FSA

Crystal structure of hen egg white lysozyme

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SEALED TUBE
Source details	OXFORD DIFFRACTION ENHANCE ULTRA
Temperature [K]	125
Detector technology	CCD
Collection date	2020-11-03
Detector	OXFORD TITAN CCD
Wavelength(s)	1.5406
Spacegroup name	P 43 21 2
Unit cell lengths	77.404, 77.404, 37.142
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	13.410 - 1.600
R-factor	0.2215
Rwork	0.219
R-free	0.26713
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6qwy
RMSD bond length	0.008
RMSD bond angle	1.515
Data reduction software	HKL-2000
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	13.400	1.630
High resolution limit [Å]	1.600	1.600
Rmerge	0.115	1.817
Rmeas	0.130	2.243
Rpim	0.057	1.286
Total number of observations	68827	2148
Number of reflections	15256	750
<I/σ(I)>	7	0.5
Completeness [%]	99.2
Redundancy	4.5	2.9
CC(1/2)	0.993	0.289

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	4.6	295	1.2 M NaCl, 100 mM Sodium Acetate, 50 mg/mL Lyzozyme, 30% Glycerol for cryoprotection