8FPM

Crystal structure of hen egg white lysozyme

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SEALED TUBE
Source details	OXFORD DIFFRACTION ENHANCE ULTRA
Temperature [K]	125
Detector technology	CCD
Collection date	2020-08-28
Detector	OXFORD TITAN CCD
Wavelength(s)	1.54056
Spacegroup name	P 43 21 2
Unit cell lengths	77.741, 77.741, 37.104
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	13.460 - 1.600
R-factor	0.24036
Rwork	0.238
R-free	0.27898
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.468
Data reduction software	HKL-2000
Data scaling software	Aimless
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	13.450	1.690
High resolution limit [Å]	1.600	1.600
Rmeas	0.152	0.964
Rpim	0.062	0.535
Total number of observations	74502	6777
Number of reflections	15389	2189
<I/σ(I)>	5.9	0.5
Completeness [%]	99.1
Redundancy	4.8	3.1
CC(1/2)	0.992	0.314

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	4.6	295	1.2 M NaCl, 100 mM Sodium Acetate, 50 mg/mL Lyzozyme, 30% Glycerol for cryoprotection