8FNA

Crystal structure of the C-terminal Fg domain of human TNR

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2013-10-08
Detector	MAR scanner 300 mm plate
Wavelength(s)	1.00
Spacegroup name	P 31 2 1
Unit cell lengths	47.423, 47.423, 203.403
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	38.080 - 1.750
R-factor	0.1619
Rwork	0.160
R-free	0.18520
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2j3f
RMSD bond length	0.013
RMSD bond angle	1.131
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.810
High resolution limit [Å]	1.750	1.750
Rmerge	0.128	0.491
Rpim	0.060	0.240
Number of reflections	27862	2576
<I/σ(I)>	10.1	2.7
Completeness [%]	99.2	94.9
Redundancy	5.7	4.6
CC(1/2)	0.985	0.845

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	10% PEG3350, 0.2 M L-proline, 100 mM HEPES sodium, pH 7.5, 5 mM calcium chloride