8FN9

Crystal structure of the C-terminal Fg domain of TNR with the single FN domain of PTPRZ

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-12-08
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.00
Spacegroup name	P 1
Unit cell lengths	48.325, 75.656, 88.322
Unit cell angles	104.41, 92.40, 91.81

Refinement procedure

Resolution	42.960 - 1.800
R-factor	0.1747
Rwork	0.174
R-free	0.21020
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB entries 2J3F & 1FNA
RMSD bond length	0.011
RMSD bond angle	0.877
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.860
High resolution limit [Å]	1.800	1.800
Rmerge	0.157	0.991
Rpim	0.062	0.517
Number of reflections	102222	10193
<I/σ(I)>	11.7	1.1
Completeness [%]	91.6	91.1
Redundancy	6.6	3.9
CC(1/2)	0.997	0.486

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	20% w/v PEG2000 MME, 200 mM potassium thiocyanate