8FM2

HIV-1 gp120 complex with CJF-III-289

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-04-17
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	71.223, 121.179, 194.626
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.660 - 2.400
R-factor	0.221
Rwork	0.219
R-free	0.26220
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.013
RMSD bond angle	1.616
Data reduction software	XDS
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.20rc3_4406)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.660	2.730
High resolution limit [Å]	2.400	2.400
Rmerge	0.558	3.697
Rpim	0.166	1.046
Number of reflections	40583	2029
<I/σ(I)>	7.7
Completeness [%]	92.6
Redundancy	13.5
CC(1/2)	0.984	0.349

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	14% to 16% (w/v) PEG 1500, 0.1 M calcium chloride, 0.1 M imidazole pH 6.5