8FG3
HRAS R97L Crystal Form 2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2010-03-13 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.0 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 39.650, 39.650, 157.616 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 28.740 - 1.490 |
R-factor | 0.2094 |
Rwork | 0.206 |
R-free | 0.24730 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3k8y |
RMSD bond length | 0.007 |
RMSD bond angle | 1.020 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.740 | 1.543 |
High resolution limit [Å] | 1.490 | 1.490 |
Rmerge | 0.050 | 0.208 |
Number of reflections | 24494 | 2387 |
<I/σ(I)> | 42.81 | 12.81 |
Completeness [%] | 99.9 | |
Redundancy | 5.6 | 5.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291.15 | PEG 3350, Calcium Acetate, Magnesium Chloride, Sodium Chloride, DTT |