8F9U
Crystal structure of Ky15.7 Fab in complex with circumsporozoite protein NPDP peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-02-17 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.033167 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 50.990, 60.696, 147.834 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.260 - 1.700 |
R-factor | 0.2089 |
Rwork | 0.208 |
R-free | 0.22950 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.003 |
RMSD bond angle | 0.617 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.19_4080) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 1.730 |
High resolution limit [Å] | 1.700 | 1.700 |
Number of reflections | 50113 | 2481 |
<I/σ(I)> | 10.3 | |
Completeness [%] | 97.4 | |
Redundancy | 5.5 | |
CC(1/2) | 0.833 | 0.794 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 0.2 M di-ammonium hydrogen phosphate, 20% PEG3350 |