8EVE

HUMAN DNA POLYMERASE ETA INSERTION COMPLEX

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-07-17
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.12723
Spacegroup name	P 61
Unit cell lengths	98.990, 98.990, 81.470
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	32.400 - 2.350
R-factor	0.1917
Rwork	0.189
R-free	0.24430
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5l1i
RMSD bond length	0.008
RMSD bond angle	1.003
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.400	2.440
High resolution limit [Å]	2.350	2.350
Rmerge	0.087
Rmeas	0.227	0.674
Rpim	0.064	0.499
Number of reflections	18885	1397
<I/σ(I)>	5.3	2.1
Completeness [%]	99.5	99.5
Redundancy	10.3	5.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	291	0.1 M MES PH 5.5, 5mM Calcium Chloride, 15-22% PEG 2000 MME