8EHG
Rabbit muscle aldolase determined using single-particle cryo-EM with Apollo camera.
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.7 |
| Detector | OTHER |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 2.24 |
| Number of Particle | 3805094 |
| Software | cryoSPARC,Leginon,CTFFIND |
| Symmetry | D2 |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
