8E0B
Crystal structure of human Sar1bT39N
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE A1 |
Synchrotron site | CHESS |
Beamline | A1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-12-05 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.9778 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 52.971, 61.814, 70.681 |
Unit cell angles | 90.00, 106.17, 90.00 |
Refinement procedure
Resolution | 36.166 - 2.212 |
R-factor | 0.1798 |
Rwork | 0.174 |
R-free | 0.23730 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 8dzm |
RMSD bond length | 0.007 |
RMSD bond angle | 1.033 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.240 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.117 | 0.492 |
Number of reflections | 20483 | 745 |
<I/σ(I)> | 12.3 | |
Completeness [%] | 93.4 | 70.7 |
Redundancy | 3.9 | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 1.2M (NH4)2SO4 and 0.1M sodium citrate, pH5.5. |