8DPU
The crystal structure of the IL-11 signalling complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-05-08 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9537 |
Spacegroup name | P 31 1 2 |
Unit cell lengths | 163.411, 163.411, 506.623 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 40.860 - 3.780 |
R-factor | 0.2765 |
Rwork | 0.276 |
R-free | 0.29510 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 8dps |
RMSD bond length | 0.006 |
RMSD bond angle | 1.204 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.275 | 4.197 |
High resolution limit [Å] | 3.779 | 3.779 |
Rmeas | 0.294 | 2.584 |
Rpim | 0.064 | 0.558 |
Number of reflections | 48027 | 2262 |
<I/σ(I)> | 10.6 | 1.5 |
Completeness [%] | 92.8 | |
Redundancy | 20.9 | |
CC(1/2) | 0.998 | 0.548 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.25 | 293 | 180 mM magnesium chloride, 15.3% PEG 3350, 100 mM potassium sodium tartrate, 90 mM sodium HEPES pH 7.25 and 1.8% tert-butanol |