8DP3

Crystal structure of coxsackievirus B3 cloverleaf RNA replication element

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-12-11
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97911
Spacegroup name	C 1 2 1
Unit cell lengths	122.173, 48.698, 144.653
Unit cell angles	90.00, 112.92, 90.00

Refinement procedure

Resolution	66.620 - 1.910
R-factor	0.2108
Rwork	0.209
R-free	0.25940
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6b3k
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX ((1.19.1))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	66.640	1.950
High resolution limit [Å]	1.900	1.900
Rmerge	0.080	0.706
Rmeas	0.080	0.850
Rpim	0.052	0.465
Number of reflections	60296	17592
<I/σ(I)>	10
Completeness [%]	97.4	89.6
Redundancy	3.3	3.1
CC(1/2)	0.997	0.743

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	298	Magnesium Acetate Tetrahydrate (0.01 M), MES Monohydrate (0.05 M), Ammonium Sulfate (2.5 M)