8DLE
Crosslinked Crystal Structure of the 8-amino-7-oxonanoate synthase, BioF, and Benzene Sulfonyl Fluoride-crypto Acyl Carrier Protein, BSF-ACP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.1 |
Synchrotron site | ALS |
Beamline | 8.2.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2021-02-18 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 94.490, 162.310, 70.970 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 53.570 | 2.382 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.081 | 0.768 |
Rmeas | 0.114 | 1.086 |
Rpim | 0.081 | 0.768 |
Number of reflections | 20184 | 1669 |
<I/σ(I)> | 7.37 | 0.47 |
Completeness [%] | 81.0 | 69.26 |
Redundancy | 1.8598 | 1.8586 |
CC(1/2) | 0.989 | 0.316 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 289.15 | 1.5 M ammonium sulfate |