8DHT

Crystal structure of a typeIII Rubisco

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F1
Synchrotron site	CHESS
Beamline	F1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-05-12
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9775
Spacegroup name	P 1 21 1
Unit cell lengths	80.329, 116.206, 83.285
Unit cell angles	90.00, 96.32, 90.00

Refinement procedure

Resolution	49.377 - 1.699
R-factor	0.1442
Rwork	0.144
R-free	0.17740
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3kdo
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.730
High resolution limit [Å]	1.699	1.699
Rmerge	0.062	0.377
Rmeas	0.074	0.452
Rpim	0.040	0.247
Number of reflections	166579	8243
<I/σ(I)>	16.5	2.087
Completeness [%]	100.0	99.9
Redundancy	3.4	3.3
CC(1/2)	0.993	0.836

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	295	26% PEG4000 (w/v), 0.2M NaAc, 0.1M Tris-HCl, pH8.0