8DHL
Tannerella forsythia beta-glucuronidase (L2)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-04-04 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.96802 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 77.983, 119.209, 209.883 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 78.770 - 2.300 |
R-factor | 0.1704 |
Rwork | 0.170 |
R-free | 0.21030 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5uj6 |
Data reduction software | XDS |
Data scaling software | Aimless (0.7.7) |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4489) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 103.660 | 103.660 | 2.340 |
High resolution limit [Å] | 2.300 | 12.390 | 2.300 |
Rmerge | 0.193 | 0.119 | 0.563 |
Rmeas | 0.210 | 0.133 | 0.610 |
Rpim | 0.081 | 0.057 | 0.234 |
Total number of observations | 575179 | 3891 | 29550 |
Number of reflections | 87551 | 673 | 4405 |
<I/σ(I)> | 7 | 10.7 | 3.3 |
Completeness [%] | 99.9 | 99.8 | 100 |
Redundancy | 6.6 | 5.8 | 6.7 |
CC(1/2) | 0.979 | 0.985 | 0.859 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.8 | 293.15 | 6% (w/v) PEG8000, 8% (v/v) ethylene glycol, 2% (v/v) glycerol, 0.1 M Tris-HCl |