8DH0
T7 RNA polymerase elongation complex with unnatural base dDs
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-1 |
Synchrotron site | SSRL |
Beamline | BL12-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-07-16 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 |
Unit cell lengths | 78.707, 86.235, 201.220 |
Unit cell angles | 89.91, 85.23, 69.50 |
Refinement procedure
Resolution | 42.030 - 2.900 |
R-factor | 0.2213 |
Rwork | 0.221 |
R-free | 0.26100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1h38 |
Data reduction software | DIALS |
Data scaling software | xia2 |
Phasing software | PHENIX (1.19) |
Refinement software | PHENIX (1.19) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 42.030 | 39.640 | 2.700 |
High resolution limit [Å] | 2.660 | 14.570 | 2.660 |
Rmerge | 0.099 | 0.035 | 5.785 |
Rmeas | 0.115 | 0.041 | 6.716 |
Rpim | 0.058 | 0.021 | 3.403 |
Total number of observations | 3232 | 24203 | |
Number of reflections | 131648 | 851 | 6456 |
<I/σ(I)> | 6.9 | 25.6 | 0.3 |
Completeness [%] | 89.7 | 94.8 | 88.9 |
Redundancy | 3.9 | 3.8 | 3.7 |
CC(1/2) | 0.997 | 0.996 | 0.234 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.1 | 298 | 10% PEG 8000, 8% glycerol, 5 mM B-mercaptoethanol, 100 mM Tris pH 8.1 |