8DAM
nbF3:nbE8:CaV beta subunit 1b complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-03-17 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 1.1159 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 80.358, 81.072, 93.332 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.690 - 2.000 |
R-factor | 0.1948 |
Rwork | 0.193 |
R-free | 0.22680 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5v2p |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.690 | 2.072 |
High resolution limit [Å] | 2.000 | 2.000 |
Number of reflections | 41850 | 4114 |
<I/σ(I)> | 17.53 | |
Completeness [%] | 98.9 | |
Redundancy | 12.7 | |
CC(1/2) | 0.999 | 0.813 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277.15 | 15-22% PEG3350, 0.15-0.25M Ammonium Sulphate |