8D0S
Human FUT9 bound to GDP and LNnT
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-07-09 |
Detector | RAYONIX MX300-HS |
Wavelength(s) | 1.0 |
Spacegroup name | I 21 3 |
Unit cell lengths | 127.160, 127.160, 127.160 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.700 - 1.370 |
R-factor | 0.1588 |
Rwork | 0.158 |
R-free | 0.18150 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 8d0o |
RMSD bond length | 0.005 |
RMSD bond angle | 0.968 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX |
Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 36.700 | 1.410 |
High resolution limit [Å] | 1.370 | 1.370 |
Rmeas | 0.116 | 2.500 |
Number of reflections | 71501 | 7124 |
<I/σ(I)> | 15.1 | 1.3 |
Completeness [%] | 99.9 | 100 |
Redundancy | 22.1 | 21.9 |
CC(1/2) | 0.999 | 0.506 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 2.0 M Ammonium sulfate, 100 mM Tris pH 8.5 |