8CTB
Human PRMT5:MEP50 structure with Fragment 3 and MTA Bound
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-06-17 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.979 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 97.983, 137.232, 178.304 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.670 - 2.610 |
R-factor | 0.2486 |
Rwork | 0.246 |
R-free | 0.28850 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7uy1 |
RMSD bond length | 0.002 |
RMSD bond angle | 0.442 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.700 | 2.640 |
High resolution limit [Å] | 2.610 | 2.610 |
Rmerge | 0.600 | |
Number of reflections | 28091 | 427 |
<I/σ(I)> | 9.4 | |
Completeness [%] | 76.1 | |
Redundancy | 2.7 | |
CC(1/2) | 0.990 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 291 | 10% PEG 8000, 100 mM Sodium Citrate 5.4, 100 mM Sodium Acetate |