8COE
complement C5 in complex with the LCP0195 nanobody
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MAX IV BEAMLINE BioMAX |
Synchrotron site | MAX IV |
Beamline | BioMAX |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-02-01 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9762 |
Spacegroup name | I 41 |
Unit cell lengths | 194.830, 194.830, 207.540 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.710 - 4.200 |
R-factor | 0.2493 |
Rwork | 0.248 |
R-free | 0.26890 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.402 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.710 | 4.350 |
High resolution limit [Å] | 4.200 | 4.200 |
Rmerge | 0.129 | 2.421 |
Number of reflections | 28237 | 2779 |
<I/σ(I)> | 11.94 | |
Completeness [%] | 99.8 | 100 |
Redundancy | 14.1 | 14.2 |
CC(1/2) | 0.999 | 0.413 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 292 | 0.025 M succinic acid pH 7.0 and 3.75% w/v polyethylene glycol 3350. |