8BUK
Structure of DDB1 bound to DS08-engaged CDK12-cyclin K
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-07-20 |
Detector | DECTRIS EIGER2 X 16M |
Wavelength(s) | 1.0 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 248.824, 248.824, 217.984 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 72.660 - 3.410 |
R-factor | 0.1862 |
Rwork | 0.185 |
R-free | 0.21020 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.004 |
RMSD bond angle | 0.784 |
Data reduction software | XDS |
Data scaling software | STARANISO |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 215.488 | 3.562 |
High resolution limit [Å] | 3.406 | 3.406 |
Number of reflections | 92889 | 4644 |
<I/σ(I)> | 15.5 | |
Completeness [%] | 91.2 | |
Redundancy | 20.8 | |
CC(1/2) | 0.999 | 0.514 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.84 M ammonium sulfate, 0.36 M ammonium citrate, 0.07 M HEPES pH 7 |