8BQL
W-formate dehydrogenase from Desulfovibrio vulgaris - Co-crystallized with Formate and Reoxidized by exposure to air for 12 min
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID30B |
Synchrotron site | ESRF |
Beamline | ID30B |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-07-06 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9686 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 64.800, 127.594, 148.622 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 96.999 - 1.906 |
Rwork | 0.188 |
R-free | 0.22510 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6sdv |
RMSD bond length | 0.004 |
RMSD bond angle | 1.278 |
Data reduction software | autoPROC |
Data scaling software | STARANISO |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 96.999 | 2.008 |
High resolution limit [Å] | 1.906 | 1.906 |
Number of reflections | 82382 | 4121 |
<I/σ(I)> | 9.086 | |
Completeness [%] | 94.5 | |
Redundancy | 10.95 | |
CC(1/2) | 0.997 | 0.620 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 26% PEG 3350, 0.1M Tris-HCl pH 8.0, 1M LiCl, 10 mM sodium formate |