8BCF
Human Brr2 Helicase Region in complex with C-tail deleted Jab1 and compound 78
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-11-12 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9184 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 99.549, 118.698, 186.917 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.250 - 2.420 |
R-factor | 0.2144 |
Rwork | 0.213 |
R-free | 0.27450 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6s8q |
RMSD bond length | 0.005 |
RMSD bond angle | 0.752 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.20_4459) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.560 |
High resolution limit [Å] | 2.420 | 2.420 |
Rmeas | 0.231 | |
Number of reflections | 85165 | 13518 |
<I/σ(I)> | 9.14 | 0.83 |
Completeness [%] | 99.9 | 99.4 |
Redundancy | 10.5 | 10.5 |
CC(1/2) | 0.997 | 0.365 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 291.15 | 0.1M Hepes-NaOH, 0.1M MgCl2, 8% PEG 3350 |