8B55
Human ADGRG4 PTX-like domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P14 (MX2) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-09-29 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9762 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 113.117, 40.243, 54.912 |
Unit cell angles | 90.00, 113.90, 90.00 |
Refinement procedure
Resolution | 51.710 - 1.360 |
R-factor | 0.1571 |
Rwork | 0.154 |
R-free | 0.18880 |
Structure solution method | SAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.046 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 51.710 | 1.380 |
High resolution limit [Å] | 1.356 | 1.360 |
Rmeas | 0.028 | 0.530 |
Rpim | 0.011 | 0.230 |
Number of reflections | 43935 | 282 |
<I/σ(I)> | 29.6 | 2.9 |
Completeness [%] | 89.7 | 20 |
Redundancy | 6.9 | |
CC(1/2) | 0.999 | 0.920 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 292.15 | 0.1 M Tris pH 8.5, 30 % PEG 4000, 0.2 M magnesium chloride |