8B4J

Rfa1-N-terminal domain in complex with phosphorylated Ddc2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-07-07
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9794
Spacegroup name	P 65 2 2
Unit cell lengths	39.996, 39.996, 282.062
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.010 - 1.580
R-factor	0.1933
Rwork	0.191
R-free	0.22940
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5omb
RMSD bond length	0.014
RMSD bond angle	1.624
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	56.400	1.640
High resolution limit [Å]	1.580	1.580
Rmerge	0.051	0.492
Number of reflections	19852	1878
<I/σ(I)>	23.8
Completeness [%]	100.0	99.8
Redundancy	6.8
CC(1/2)	0.990

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	295	10 mM Zinc Chloride, 0.1M MES, pH 6.0, 20% w/v PEG 6000