8AXN
Inner membrane ring and secretin N0 N1 domains of the type 3 secretion system of Shigella flexneri
This is a non-PDB format compatible entry.
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.7 |
| Detector | FEI FALCON II (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.34 |
| Software | RELION,EPU,CTFFIND,UCSF Chimera,PHENIX |
| Symmetry | C8 |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
