8AEH

X-ray structure of Canis familiaris Odorant Binding Protein 1

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-06-03
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.978565
Spacegroup name	P 41 21 2
Unit cell lengths	88.221, 88.221, 37.517
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.110 - 3.000
R-factor	0.286
Rwork	0.285
R-free	0.30110
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3eyc
RMSD bond length	0.010
RMSD bond angle	1.466
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.110	3.180
High resolution limit [Å]	3.000	3.000
Rmerge	0.066	0.188
Rmeas	0.067	0.192
Rpim	0.014	0.038
Number of reflections	3242	512
<I/σ(I)>	32.5	14.5
Completeness [%]	100.0	100
Redundancy	25
CC(1/2)	0.990	0.997

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	20% PEG 4000, 0.2 M CaCl2 in 0.1 M pH 8.5 Tris buffer