8A58
X-ray structure of TRIM21 RING E3 ligase in complex with E2 enzyme Ube2W
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID2 |
Synchrotron site | ESRF |
Beamline | ID2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-11-16 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.984004 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 62.820, 75.482, 63.835 |
Unit cell angles | 90.00, 119.31, 90.00 |
Refinement procedure
Resolution | 29.460 - 2.250 |
R-factor | 0.2174 |
Rwork | 0.215 |
R-free | 0.25880 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5olm |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.460 | 2.330 |
High resolution limit [Å] | 2.250 | 2.250 |
Number of reflections | 23652 | 2357 |
<I/σ(I)> | 16.49 | |
Completeness [%] | 95.3 | |
Redundancy | 3.3 | |
CC(1/2) | 0.999 | 0.859 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 290 | 0.1 M Bicine pH 9.0, 5% PEG 6000, 0.1M TCEP hydrochloride |