8A0W

Crystal structure of the HigA2 antitoxin in complex with operator DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-05-24
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97911
Spacegroup name	P 32 2 1
Unit cell lengths	94.030, 94.030, 123.720
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.259 - 2.334
R-factor	0.1752
Rwork	0.174
R-free	0.19940
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5j9i
RMSD bond length	0.008
RMSD bond angle	0.959
Data reduction software	autoXDS (v1.0)
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.260	2.470
High resolution limit [Å]	2.330	2.330
Rmeas	0.078	0.793
Number of reflections	27228	4150
<I/σ(I)>	18.15	2.02
Completeness [%]	99.2
Redundancy	10.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	0.2 M sodium chloride, 0.1 M Na HEPES pH 7.5, 12%(w/v) PEG 8000