7ZWF
Pfs48/45 bound to scFv fragment of monoclonal antibody 32F3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-07-01 |
Detector | DECTRIS EIGER2 X 16M |
Wavelength(s) | 0.97949 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 81.826, 126.115, 146.787 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.910 - 2.132 |
R-factor | 0.2762 |
Rwork | 0.276 |
R-free | 0.28640 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6h5n |
RMSD bond length | 0.008 |
RMSD bond angle | 0.990 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.4 (20-OCT-2021)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 73.390 | 2.170 |
High resolution limit [Å] | 2.130 | 2.130 |
Rpim | 0.037 | 2.260 |
Number of reflections | 586552 | 28745 |
<I/σ(I)> | 11.2 | |
Completeness [%] | 99.8 | |
Redundancy | 13.8 | |
CC(1/2) | 0.999 | 0.384 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.1M MES pH6.0, 0.2M CaCl2, 20% PEG6000 |