7ZLQ
Crystal structure of ADAR1-dsRBD3 dimer in complex with dsRNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-09-12 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.9999 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 91.294, 91.294, 207.527 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.580 - 2.800 |
| R-factor | 0.2349 |
| Rwork | 0.231 |
| R-free | 0.26600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7zj1 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.878 |
| Data reduction software | XDS |
| Data scaling software | STARANISO |
| Phasing software | PHASER (2.8.2) |
| Refinement software | REFMAC (5.8.0) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 44.580 | 44.580 | 2.990 |
| High resolution limit [Å] | 2.800 | 8.890 | 2.800 |
| Rmerge | 0.163 | 0.031 | 2.663 |
| Rmeas | 0.167 | 0.032 | 2.739 |
| Rpim | 0.038 | 0.010 | 0.633 |
| Number of reflections | 10044 | 502 | 503 |
| <I/σ(I)> | 16.6 | 61.8 | 1.2 |
| Completeness [%] | 94.9 | 100 | 88.6 |
| Redundancy | 19.2 | 16.1 | 18.3 |
| CC(1/2) | 1.000 | 1.000 | 0.517 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 50 mM sodium cacodylate, 5% (w/v) PEG 4000, 30 mM CaCl2, 230 mM KCl |
