7Z0E

Crystal structure of the M state of bacteriorhodopsin at 1.22 Angstrom resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-1
Synchrotron site	ESRF
Beamline	ID14-1
Temperature [K]	100
Detector technology	CCD
Collection date	2006-07-10
Detector	ADSC QUANTUM 210
Wavelength(s)	0.934
Spacegroup name	P 63
Unit cell lengths	60.890, 60.890, 109.230
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.445 - 1.220
R-factor	0.1865
Rwork	0.182
R-free	0.19510
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1c3w
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.445	30.445	1.250
High resolution limit [Å]	1.220	5.460	1.220
Rmerge	0.066	0.029	2.209
Rmeas	0.071	0.032	2.774
Total number of observations	427782
Number of reflections	66766	772	4327
<I/σ(I)>	13.75	58.7	0.44
Completeness [%]	98.1	98	86.1
Redundancy	6.407	8.65	2.292
CC(1/2)	0.999	0.999	0.173

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE		293	Na2HPO4 (5%) and KH2PO4 (95%)