7Z0C

Crystal structure of the K state of bacteriorhodopsin at 1.53 Angstrom resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-2
Synchrotron site	ESRF
Beamline	ID14-2
Temperature [K]	100
Detector technology	CCD
Collection date	2010-02-05
Detector	ADSC QUANTUM 4
Wavelength(s)	0.933
Spacegroup name	P 63
Unit cell lengths	60.990, 60.990, 110.080
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	38.110 - 1.530
R-factor	0.1499
Rwork	0.149
R-free	0.17090
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1c3w
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	38.110	38.110	1.890
High resolution limit [Å]	1.530	8.230	1.840
Rmerge	0.067	0.016	1.567
Rmeas	0.079	0.018	2.157
Number of reflections	30368	199	598
<I/σ(I)>	13.5	68.65	0.31
Completeness [%]	85.0	82.6	40.2
Redundancy	3.059	4.085	1.386
CC(1/2)	0.998	1.000

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE		293	Na2HPO4 (5%) and KH2PO4 (95%)