7YWW
Monocot chimeric jacalin JAC1 from Oryza sativa: lectin domain (crystal form 2)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-11-24 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.919 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 80.320, 99.630, 93.740 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.870 - 1.400 |
R-factor | 0.17 |
Rwork | 0.169 |
R-free | 0.19460 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4pif |
RMSD bond length | 0.013 |
RMSD bond angle | 1.190 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 52.020 | 1.440 |
High resolution limit [Å] | 1.400 | 1.400 |
Number of reflections | 71961 | 5441 |
<I/σ(I)> | 9.65 | 0.85 |
Completeness [%] | 97.2 | 99.5 |
Redundancy | 3.74 | 3.74 |
CC(1/2) | 0.999 | 0.501 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.3 | 293 | PEG 3350, ammonium iodide, potassium phosphate, N-acetylgalactosamine |