7YWG
Monocot chimeric jacalin JAC1 from Oryza sativa: lectin domain (crystal form 1)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-11-16 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97879 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 34.930, 63.510, 105.880 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 54.460 - 1.100 |
R-factor | 0.1568 |
Rwork | 0.155 |
R-free | 0.18800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4pif |
RMSD bond length | 0.007 |
RMSD bond angle | 1.031 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 54.460 | 1.130 |
High resolution limit [Å] | 1.100 | 1.100 |
Number of reflections | 87556 | 3988 |
<I/σ(I)> | 5.45 | 0.85 |
Completeness [%] | 90.9 | 56.7 |
Redundancy | 2.25 | 1.35 |
CC(1/2) | 0.988 | 0.612 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | PEG 3350, MES, potassium phosphate, sodium chloride |