7YCU

Heterotetramer of Antitoxin PrpA together with Toxin PrpT from Pseudoalteromonas rubra

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-01-16
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.979183
Spacegroup name	P 1 21 1
Unit cell lengths	44.782, 83.085, 56.489
Unit cell angles	90.00, 91.66, 90.00

Refinement procedure

Resolution	44.760 - 1.790
R-factor	0.1691
Rwork	0.168
R-free	0.20000
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.414
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1-4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.760	1.857
High resolution limit [Å]	1.790	1.790
Rmerge	0.084	0.791
Rmeas	0.091	0.872
Rpim	0.035	0.359
Number of reflections	38264	3740
<I/σ(I)>	16.7	2.9
Completeness [%]	98.6	97.22
Redundancy	6.7	5.7
CC(1/2)	0.999	0.809

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	289	0.1 M Sodium cacodylate pH 6.0, 15 % w/v PEG 4000