7Y5Y
X-ray Structure of Stay-Green (SGR) from Anaerolineae bacterium.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44XU |
Synchrotron site | SPring-8 |
Beamline | BL44XU |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-07-23 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.90000 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 80.331, 80.331, 224.803 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.770 - 1.850 |
R-factor | 0.2187 |
Rwork | 0.218 |
R-free | 0.23920 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | AlphaFold2 predicted structure of rice SGR (UniProt: Q652K1) |
RMSD bond length | 0.009 |
RMSD bond angle | 1.328 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER (1.18.2_3874) |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.740 | 1.840 |
High resolution limit [Å] | 1.750 | 1.750 |
Rmerge | 0.061 | 1.158 |
Number of reflections | 44094 | 6906 |
<I/σ(I)> | 14.07 | |
Completeness [%] | 99.4 | |
Redundancy | 6.5 | |
CC(1/2) | 0.999 | 0.829 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 298 | 100 mM Tris-HCl (pH 8.5), 200 mM Ammonium phosphate monobasic and 50% (v/v) 2-Methyl-2,4-pentanediol |