7Y54
Crystal structure of sDscam Ig1 domain, isoform alpha1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-12-05 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9793 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 67.395, 40.728, 41.482 |
Unit cell angles | 90.00, 94.18, 90.00 |
Refinement procedure
Resolution | 20.686 - 1.787 |
R-factor | 0.1827 |
Rwork | 0.180 |
R-free | 0.22600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6zr7 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.830 |
High resolution limit [Å] | 1.787 | 1.787 |
Number of reflections | 10665 | 508 |
<I/σ(I)> | 8.6 | |
Completeness [%] | 100.0 | |
Redundancy | 6.4 | |
CC(1/2) | 0.960 | 0.940 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 289 | 40% isopropanol, 100 mM Imidazole pH 6.5, 15% PEG 8000 |