7Y4F
bacterial DPP4
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-07-12 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9783 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 95.800, 148.857, 137.125 |
Unit cell angles | 90.00, 104.02, 90.00 |
Refinement procedure
Resolution | 46.364 - 1.918 |
R-factor | 0.1691 |
Rwork | 0.168 |
R-free | 0.19330 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1r9n |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.364 | 1.950 |
High resolution limit [Å] | 1.918 | 1.920 |
Number of reflections | 283945 | 3107 |
<I/σ(I)> | 12.6 | |
Completeness [%] | 99.4 | |
Redundancy | 3.4 | |
CC(1/2) | 0.984 | 0.512 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 9 | 291.15 | 0.1M BICINE pH 9.0, 10% w/v PEG 20,000 and 3% 6-aminohexanoic acid solution |