7XYO
Crystal Structure of a M61 aminopeptidase family member from Myxococcus fulvus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-09-19 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.979 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 96.679, 113.278, 132.345 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.110 - 2.700 |
R-factor | 0.2188 |
Rwork | 0.217 |
R-free | 0.24960 |
Structure solution method | MOLECULAR REPLACEMENT |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.19.1_4122) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.750 |
High resolution limit [Å] | 2.700 | 7.320 | 2.700 |
Rmerge | 0.155 | 0.066 | 0.905 |
Rmeas | 0.168 | 0.072 | 0.986 |
Rpim | 0.065 | 0.029 | 0.386 |
Total number of observations | 266197 | ||
Number of reflections | 40390 | 2205 | 1971 |
<I/σ(I)> | 4.3 | ||
Completeness [%] | 99.9 | 99.7 | 99.6 |
Redundancy | 6.6 | 6.1 | 6.4 |
CC(1/2) | 0.996 | 0.835 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 289 | Sodium acetate, Tris-HCl, PEG10000 |