7XXY

Macaca mulatta galectin-10/Charcot-Leyden crystal protein with lactose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-01-12
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97930
Spacegroup name	P 65 2 2
Unit cell lengths	48.609, 48.609, 256.265
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.470 - 1.920
R-factor	0.1774
Rwork	0.174
R-free	0.20900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5xrg
Refinement software	PHENIX (1.17_3644)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.470	1.970
High resolution limit [Å]	1.920	1.920
Rmerge	0.103	0.741
Number of reflections	14830	941
<I/σ(I)>	21.1
Completeness [%]	99.9
Redundancy	17.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	PEG