7XS4
Crystal structure of URT1 in complex with AAAU RNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-07-06 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.979 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 63.164, 63.264, 81.387 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.257 - 1.846 |
R-factor | 0.1746 |
Rwork | 0.173 |
R-free | 0.20150 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6l3f |
Data reduction software | HKL-2000 |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 1.880 |
High resolution limit [Å] | 1.846 | 1.850 |
Rmerge | 0.078 | |
Number of reflections | 28694 | 2803 |
<I/σ(I)> | 24.08 | |
Completeness [%] | 100.0 | |
Redundancy | 10.6 | |
CC(1/2) | 1.000 | 0.887 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1M sodium HEPES, pH 7.5, 27% PEG 600 |