7X2J
Crystal structure of nanobody Nb70 with SARS-CoV RBD
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-11-13 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.987 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 108.413, 108.413, 94.119 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 33.240 - 2.400 |
R-factor | 0.1987 |
Rwork | 0.197 |
R-free | 0.23480 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2ajf |
RMSD bond length | 0.008 |
RMSD bond angle | 0.945 |
Data reduction software | HKL-3000 (7.21) |
Data scaling software | HKL-3000 (7.21) |
Phasing software | PHASER (2.7.0) |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 33.240 | 2.486 |
High resolution limit [Å] | 2.400 | 2.400 |
Number of reflections | 25379 | 2496 |
<I/σ(I)> | 25.45 | |
Completeness [%] | 99.8 | |
Redundancy | 17.9 | |
CC(1/2) | 0.996 | 0.611 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.4 | 291.15 | 0.2M Ammonium sulfate, 0.1M Bis-Tris pH 4.4, 21% w/v Polyethylene glycol 3350 |