7X0M
Crystal structure of sugar binding protein CbpB complexed wtih cellopentaose from Clostridium thermocellum
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-04-02 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.979 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 72.320, 46.510, 119.380 |
Unit cell angles | 90.00, 107.61, 90.00 |
Refinement procedure
Resolution | 38.555 - 2.000 |
R-factor | 0.1795 |
Rwork | 0.178 |
R-free | 0.20910 |
Structure solution method | SAD |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 38.555 | 38.555 | 2.050 |
High resolution limit [Å] | 2.000 | 8.940 | 2.000 |
Rmerge | 0.118 | 0.053 | 0.466 |
Rmeas | 0.162 | 0.074 | 0.633 |
Total number of observations | 159133 | ||
Number of reflections | 83500 | 952 | 6481 |
<I/σ(I)> | 7.12 | 12.67 | 3.98 |
Completeness [%] | 83.3 | 84.3 | 87.8 |
Redundancy | 1.906 | 1.938 | 2.005 |
CC(1/2) | 0.981 | 0.985 | 0.530 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 291 | 0.1 M Sodium acetate, pH 4.5, 20% w/v Polyethylene glycol 3350 |